Increased accumulation of the lipophilic cation tetraphenylphosphonium by cyclopiazonic acid-treated renal epithelial cells

Journal Article · Tue Jan 01 00:00:00 EST 1985 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398509530704· OSTI ID:6297589

Riley, R T; Norred, W P; Dorner, J W; Cole, R J

Pig kidney renal epithelial cells (LLC-PK1) in culture were used to determine the effects of cyclopiazonic acid (CPA) on the uptake of the transmembrane potential probe, (TH)tetraphenylphosphonium bromide (TPP ). CPA had a significant stimulatory effect on TPP accumulation, which occurred in a dose-related manner. TPP accumulation in the presence of CPA was significantly reduced by high-potassium media (HK) and carbonylcyanide m-chlorophenylhydrazone (CCCP), but neither HK nor the protonophore CCCP, could completely abolish the stimulatory effect of CPA. The apparent transmembrane potential difference, calculated based on the difference in accumulation of TPP in low-potassium and HK media, ranged from -55.9 to -85.7 mV for control cells and -89.4 to -109.0 mV for CPA-treated cells (20 mg CPA/I). The mechanism of CPA stimulation of TPP accumulation was not known. However, it was hypothesized that the effect could be a result of alterations in ion pumps or altered membrane permeability.

• Research Organization: Dept. of Agriculture, Athens, GA
• OSTI ID: 6297589
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 6
• Country of Publication: United States
• Language: English

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