Heme biosynthesis and drug metabolism in mice with hereditary hemolytic anemia

Journal Article · Sat Feb 10 00:00:00 EST 1979 · J. Biol. Chem.; (United States)

OSTI ID:6296061

Sassa, S ^[1]; Kappas, A; Bernstein, S E; Alvares, A P

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1. Rockefeller Univ., New York, NY

Mice homozygous for the nb (normoblastosis) gene have severe hemolytic anemia characterized by increased catabolism were measured in the tissues of homozygous (nb/nb), heterozygous (+/nb), and control (+/+) mice generated on the same genetic background. The functional capacity of the microsomal hemeprotein, cytochrome P-450, was also determined in the livers of these animals. Mice homozygous for the nb gene defect had a marked increase in protoporphyrin content, delta-aminolevulinic acid (ALA)-dehydratase, and uroporphyrinogen I (URO)-synthase activities in erythrocytes. Lesser increases were observed in liver and spleen of nb/nb mice. The homozygous mice also had a marked increase in microsomal heme oxygenase activity in the liver, kidney, and spleen compared to normal controls. The increase in heme oxygenase activity is attributable to a higher specific activity per mg of microsomal protein in the case of the liver and the kidney and to the marked organamegaly in the case of the spleen.

• OSTI ID: 6296061
• Journal Information: J. Biol. Chem.; (United States), Vol. 254:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANEMIAS
GENETICS
DRUGS
METABOLISM
HEME
BIOSYNTHESIS
CYTOCHROMES
ENZYMES
ERYTHROCYTES
EXPERIMENTAL DATA
GRAPHS
HEMOGLOBIN
ISOLATED VALUES
MICE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
DATA
DATA FORMS
DISEASES
GLOBIN
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
MAMMALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RODENTS
SYMPTOMS
SYNTHESIS
VERTEBRATES
550200* - Biochemistry
550400 - Genetics