Simple radiometric assay for methotrexate and other folate antagonists. [Tritium tracer technique]
Journal Article
·
· J. Lab. Clin. Med.; (United States)
OSTI ID:6295510
A rapid radiometric assay for the folate antagonist MTX, based on the inhibition of chicken liver dihydrofolate reductase activity, has been developed. In the system the inhibition by MTX of the reduction of tritiated FH/sub 2/ is standardized and used to quantitate this antagonist in plasma of subjects receiving high-dose MTX therapy. The assay permits the detection of plasma MTX levels to 5 x 10/sup -9/M with a coefficient of variation of 15%. The predominating circulating folate, 5 methyltetrahydrofolate, or the rescue agent leucovorin (5 formyltetrahydrofolate) do not significantly interfere with the assay at concentrations attainable with current rescue programs. Analyses of multiple plasma samples with the present assay show close agreement with the competitive protein-binding assay.
- OSTI ID:
- 6295510
- Journal Information:
- J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 93:3; ISSN JLCMA
- Country of Publication:
- United States
- Language:
- English
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Characterization of a folate-induced hypermotility response after bilateral injection into the rat nucleus accumbens
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·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
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OSTI ID:5090149
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Thesis/Dissertation
·
Tue Dec 31 23:00:00 EST 1985
·
OSTI ID:6619052
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIMETABOLITES
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DATA
DATA FORMS
DRUGS
ENZYMES
EXPERIMENTAL DATA
GRAPHS
HYDROGEN ISOTOPES
INFORMATION
INHIBITION
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEDICINE
METHOTREXATE
NUCLEAR MEDICINE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
OXIDOREDUCTASES
RADIOASSAY
RADIOISOTOPES
RADIOLOGY
RADIOTHERAPY
THERAPY
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIMETABOLITES
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DATA
DATA FORMS
DRUGS
ENZYMES
EXPERIMENTAL DATA
GRAPHS
HYDROGEN ISOTOPES
INFORMATION
INHIBITION
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEDICINE
METHOTREXATE
NUCLEAR MEDICINE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
OXIDOREDUCTASES
RADIOASSAY
RADIOISOTOPES
RADIOLOGY
RADIOTHERAPY
THERAPY
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES