Actions of vasoactive intestinal peptide and secretin on chief cells prepared from guinea pig stomach

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:6294045

Sutliff, V E; Raufman, J P; Jensen, R T; Gardner, J D

Vasoactive intestinal peptide and secretin increased cellular cAMP and pepsinogen secretion in dispersed chief cells from guinea pig gastric mucosa. With each peptide there was a close correlation between the dose-response curve for changes in cellular cAMP and that for changes in pepsinogen secretion. Vasoactive intestinal peptide- (10-28) and secretin- (5-27) had no agonist activity and antagonized the actions of vasoactive intestinal peptide and secretin on cellular cAMP and pepsinogen secretion. Studies of binding of SVI-vasoactive intestinal peptide and of SV-secretin indicated that gastric chief cells possess four classes of binding sites for vasoactive intestinal peptide and secretin and that occupation of two of these classes of binding sites correlates with the abilities of vasoactive intestinal peptide and secretin to increase cellular cAMP and pepsinogen secretion. What function, in any, is mediated by occupation by the other two classes of binding sites remains to be determined.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6294045
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID PROTEINASES
ALKALI METAL COMPOUNDS
AMP
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GASTRIC ACID
GASTROINTESTINAL TRACT
HALIDES
HALOGEN COMPOUNDS
HORMONES
HYDROLASES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MATERIALS
MEMBRANES
MUCOUS MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPSIN
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHORS
PHYSIOLOGY
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
SECRETIN
SECRETION
SODIUM COMPOUNDS
SODIUM IODIDES
STOMACH
TRACER TECHNIQUES