Isolation and identification of previtamin D/sub 3/ from the skin of rats exposed to ultraviolet irradiation

Journal Article · Mon Mar 19 23:00:00 EST 1979 · Biochemistry; (United States)

OSTI ID:6293891

Holick, M F ^[1]; Richtand, N M; McNeill, S C; Holick, S A; Frommer, J E; Henley, J W; Potts, Jr, J T

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1. Massachusetts General Hospital, Boston

The process of the photolytic activation of vitamin D precursor(s) in the skin has been elucidated by a detailed analysis of the products formed after ultraviolet light exposure. The photolytic product isolated from the skin of rats exposed to ultraviolet irradiation was identified as previtamin D/sub 3/ by several criteria including its characteristic ultraviolet absorption spectrum, mass spectrum, and thermal isomerization to vitamin D/sub 3/, which itself was identified also by mass spectroscopy. Vitamin D/sub 3/ per se was not formed by ultraviolet irradiation-vitamin D/sub 3/ arises exclusively from the thermal conversion of previtamin D/sub 3/. Detectable amounts of lumisterol/sub 3/ or tachysterol/sub 3/ were not seen.

• OSTI ID: 6293891
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 18:6; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHOLECALCIFEROL
DATA
DATA FORMS
DECOMPOSITION
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GRAPHS
INFORMATION
MAMMALS
NUMERICAL DATA
ORGANS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
SKIN
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
VITAMIN D
VITAMINS