Two subpopulations of stem cells for T cell lineage
An assay system for the stem cell that colonizes the thymus and differentiates into T cells was developed, and by using this assay system the existence of two subpopulations of stem cells for T cell lineage was clarified. Part-body-shielded and 900-R-irradiated C57BL/6 (H-2b, Thy-1.2) recipient mice, which do not require the transfer of pluripotent stem cells for their survival, were transferred with cells from B10 X Thy-1.1 (H-2b, Thy-1.1) donor mice. The reconstitution of the recipient's thymus lymphocytes was accomplished by stem cells in the donor cells and those spared in the shielded portion of the recipient that competitively colonize the thymus. Thus, the stem cell activity of donor cells can be evaluated by determining the proportion of donor-type (Thy-1.1+) cells in the recipient's thymus. Bone marrow cells were the most potent source of stem cells. By contrast, when the stem cell activity was compared between spleen and bone marrow cells of whole-body-irradiated (800 R) C57BL/6 mice reconstituted with B10 X Thy-1.1 bone marrow cells by assaying in part-body-shielded and irradiated C57BL/6 mice, the activity of these two organs showed quite a different time course of development. The results strongly suggest that the stem cells for T cell lineage in the bone marrow comprise at least two subpopulations, spleen-seeking and bone marrow-seeking cells.
- Research Organization:
- Kyoto Univ., Japan
- OSTI ID:
- 6293054
- Journal Information:
- J. Immunol.; (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
CELL DIFFERENTIATION
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BIOLOGICAL RADIATION EFFECTS
BONE MARROW
BONE MARROW CELLS
PARTIAL BODY IRRADIATION
RADIATION CHIMERAS
SPLEEN
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STEM CELLS
THYMOCYTES
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
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BODY
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
IRRADIATION
LEUKOCYTES
MAMMALS
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MOSAICISM
ORGANS
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560151* - Radiation Effects on Animals- Man