Further characterization of benzodiazepine receptor differences in long-sleep and short-sleep mice
Molecular and conformational characteristics of benzodiazepine (BZ) receptors in cortex and cerebellum from long-sleep and mice were investigated using heat inactivation and beta-carboline competition techniques. To investigate differences in the allosteric coupling between GABA and BZ receptors, the protection of BZ receptors from heat inactivation, by GABA, was also evaluated. The two genotypes do not differ in the affinity or number of BZ receptors in the cortex or cerebellum. They do, however, appear to differ in the molecular structure and/or regulation of the conformational state of the receptor in the cortex, as indicated by a greater sensitivity of LS mice to both heat inactivation and beta-carboline competition of /sup 3/H-flunitrazepam (FNZ) binding in this region. Evidence for differences in the nature of coupling between GABA and BZ receptors is provided by the finding in that in both regions, GABA protected BZ receptors from inactivation to a greater degree in LS mice. The relationship between these differences and the multiplicity of expression of BZ receptors is discussed.
- Research Organization:
- Univ. of Colorado, Boulder (USA)
- OSTI ID:
- 6292612
- Journal Information:
- Life Sci.; (United States), Vol. 43:15
- Country of Publication:
- United States
- Language:
- English
Similar Records
GABA/benzodiazepine receptor complex in long-sleep and short-sleep mice
Benzodiazepine agonist and inverse agonist actions on GABAA receptor-operated chloride channels. II. Chronic effects of ethanol
Related Subjects
AMINOBUTYRIC ACID
BIOLOGICAL FUNCTIONS
RECEPTORS
CHEMICAL COMPOSITION
TRANQUILIZERS
BRAIN
HEAT
MICE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
DRUGS
ENERGY
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
PSYCHOTROPIC DRUGS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques