Acquisition of iron from transferrin regulates reticulocyte heme synthesis

Journal Article · Mon Nov 25 04:00:00 EST 1985 · J. Biol. Chem.; (United States)

OSTI ID:6292200

Ponka, P; Schulman, H M

Fe-salicylaldehyde isonicotinoylhydrazone (SIH), which can donate iron to reticulocytes without transferrin as a mediator, has been utilized to test the hypothesis that the rate of iron uptake from transferrin limits the rate of heme synthesis in erythroid cells. Reticulocytes take up VZFe from (VZFe)SIH and incorporate it into heme to a much greater extent than from saturating concentrations of (VZFe)transferrin. Also, Fe-SIH stimulates (2- UC)glycine into heme when compared to the incorporation observed with saturating levels of Fe-transferrin. In addition, delta-aminolevulinic acid does not stimulate VZFe incorporation into heme from either (VZFe)transferrin or (VZFe)SIH but does reverse the inhibition of VZFe incorporation into heme caused by isoniazid, an inhibitor of delta-aminolevulinic acid synthase. Taken together, these results suggest the hypothesis that some step(s) in the pathway of iron from extracellular transferrin to intracellular protoporphyrin limits the overall rate of heme synthesis in reticulocytes.

• Research Organization: Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec
• OSTI ID: 6292200
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 27; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOLEVULINIC ACID
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMICROBIAL AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENZYME INHIBITORS
ENZYMES
ERYTHROCYTES
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
GLYCINE
HEME
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDRAZIDES
INTERMEDIATE MASS NUCLEI
IRON
IRON 59
IRON ISOTOPES
ISONIAZID
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGASES
MAMMALS
MATERIALS
METALLOPROTEINS
METALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RABBITS
RADIOISOTOPES
REACTION KINETICS
RETICULOCYTES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERRIN
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES