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Intracellular transport of endocytosed chylomicron (TH)retinyl ester in rat liver parenchymal cells. Evidence for translocation of a (TH)retinoid from endosomes to endoplasmic reticulum

Journal Article · · J. Biol. Chem.; (United States)
OSTI ID:6292003
; ; ; ;

The intracellular transport of chylomicron remnants labeled with (TH)retinyl ester was studied in rat liver parenchymal cells by means of subcellular fractionation in Nycodenz and sucrose density gradients. The data presented indicate that endocytosed chylomicron remnant (TH)retinyl ester initially is located in low density endosomes. Radioactivity is subsequently transferred to a denser vesicle. Equilibrium as well as rate zonal centrifugation suggest that this denser (TH) retinoid-containing vesicle may represent endoplasmic reticulum. We have compared the intracellular transport of chylomicron remnant (TH)retinyl ester and SVI-asialofetuin. The receptor-mediated endocytosis of asialoglycoproteins in rat liver parenchymal cells is a thoroughly studied system. Our results suggest that the (TH) retinoid and SVI-asialofetuin follow the same path initially to the endosomes. After transit in endosomes, the intracellular transport differs. While asialofetuin is transported to the lysosomes, the retinoid is probably transferred to the endoplasmic reticulum.

Research Organization:
Univ. of Oslo, Norway
OSTI ID:
6292003
Journal Information:
J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 25; ISSN JBCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BODY
CELL CONSTITUENTS
CHYLOMICRONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENDOPLASMIC RETICULUM
ESTERS
GLANDS
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIVER
LYSOSOMES
MAMMALS
MEMBRANE TRANSPORT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RADIOISOTOPES
RATS
RODENTS
TRACER TECHNIQUES
TRANSLOCATION
TRITIUM COMPOUNDS
VERTEBRATES