Effects of CaS and furosemide on Cl transport and O2 uptake in rat parotid acini
Stimulation-induced changes in Cl content and O2 consumption of collagenase-isolated rat parotid acini were measured. In <10 s, carbachol caused a net Cl efflux, corresponding to approx.50% of the Cl content, and increased the O2 uptake by 100%. The increase was inhibitable by ouabain and was dependent on the presence of extracellular CaS . Furosemide reduced the unstimulated TWCl uptake and prevented the reuptake of Cl after carbachol-induced release. This suggests that a cotransport system is operating in both the unstimulated and stimulated states. Furthermore, furosemide inhibited the stimulated ouabain-sensitive OS uptake. Raising intracellular CaS by the calcium ionophore A23187 evoked the same pattern of Cl loss and O2 uptake as carboachol. Our results ae compatible with the following hypothesis. Carbachol raises intracellular CaS , causing an increased Cl permeability of the luminal membrane, resulting in a net Cl efflux. A subsequently enhanced influx of Cl and Na via a furosemide-sensitive cotransport system increases intracellular Na . This stimulates the Na -K -ATPase and thereby the OS consumption.
- Research Organization:
- The Panum Institute, Copenhagen, Denmark
- OSTI ID:
- 6291953
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 251:2
- Country of Publication:
- United States
- Language:
- English
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AMINES
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CHLORINE 36
SECRETION
OXYGEN
METABOLISM
ACETYLCHOLINE
CARBON 14 COMPOUNDS
CHLORINE COMPOUNDS
OUABAIN
RATS
SACCHAROSE
SALIVARY GLANDS
ALKALINE EARTH METAL COMPOUNDS
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHLORINE ISOTOPES
DISACCHARIDES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ESTERS
GLANDS
GLYCOSIDES
HALOGEN COMPOUNDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
NEUROREGULATORS
NONMETALS
NUCLEI
ODD-ODD NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RODENTS
SACCHARIDES
STROPHANTHINS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
551001* - Physiological Systems- Tracer Techniques