Application of continuous annular chromatography to size-exclusion separations

Conference · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6287773

Sisson, W G; Begovich, J M; Byers, C H; Scott, C D

Continuous multicomponent liquid chromatographic separations have been achieved by using a slowly rotating annular bed of sorbent material. Feed is continuously introduced at a stationary point at the top of the bed while eluent flows over the remainder of the annulus. The rotation of the sorbent bed coupled with the simultaneous elution chromatography causes the separated components of the feed streams to appear as helical bands, each of which has a characteristic, stationary exit point. The concept has been developed using continuous annular chromatography units ranging from 50 to 450 mm in diameter and operating between 7 and 1300 kPa. To illustrate size-exclusion chromatography on a continuous basis, the separation of a polysaccharide, Blue Dextran, and a much smaller molecule, CoCl/sub 2/, on Sephadex G15, was studied over a wide range of feed rates, feed concentrations, rotation rates, and eluent velocities. Plate theory, modified for application to continuous chromatography, provided an excellent means of modeling the experimental results.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6287773
• Report Number(s): CONF-870802-10; ON: DE87014406
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
ADSORBENTS
CARBOHYDRATES
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
COBALT CHLORIDES
COBALT COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
MATHEMATICAL MODELS
MOTION
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
POLYMERS
POLYSACCHARIDES
RESINS
ROTATION
SACCHARIDES
SEPARATION PROCESSES
TRANSITION ELEMENT COMPOUNDS