Charcterization of atrial natriuretic peptide receptors (ANP-R) in rat kidney and lung tissues

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6286196

Tallerico-Melnyk, T; Yu, H; Flynn, T G; Yip, C C

The ability of several rat ANP analogues to compete with SVI-ANP(1-28) for binding to plasma membranes of rat kidney cortex (RKPM) and rat lung (RLPM) was examined. Their ability to compete on RKPM was: ANP(1-28)>pro-ANP>ANP(5-28)>ANP(5-27), ANP(5-25) being inactive. Conversely, the potency of the analogues on RLPM was: ANP(5-28)>ANP(5-27)>ANP(1-28)>ANP-(5-25), pro-ANP being unable to compete. The stimulation of particulate guanylate cyclase by these peptides paralleled their ability to compete. Truncation of the C-terminal therefore decreases the binding of the peptide to RKPM. In contrast, the N-terminal seems to be important for interaction with ANP-R on RLPM. ANP-R were photolabeled with SVI-iodo-azidosalicylyl-ANP(1-28) (ASA-ANP) or azidobenzoyl- SVI-ANP(1-28) (AB-ANP) in which the C-terminal tyrosine is iodinated. In ASA-ANP, the iodine is located on the benzene ring. ASA-ANP identified a protein of approx.140 kDa in RKPM. AB-ANP recognized an additional protein of approx.120 kDa. The bulkier N-terminal of the ASA-ANP seems to hinder the binding of the analogue to the approx.120-kDa protein. In RLPM only the approx.120-kDa protein was detected by AB-ANP. The approx.140-kDa receptor may be unique to the kidney. ANF-R in RKPM and RLPM respectively, appear to interact with different domains of ANP suggesting the existence of two forms of the ANP-R.

• Research Organization: Univ., of Toronto, Ontario
• OSTI ID: 6286196
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CYCLASES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LUNGS
LYASES
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
TRACER TECHNIQUES
VERTEBRATES