Asbestos-associated chromosomal changes in human mesothelial cells

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.11.3884· OSTI ID:6285955

Lechner, J F; Tokiwa, T; LaVeck, M; Benedict, W F; Banks-Schlegel, S; Yeager, H Jr; Banerjee, A; Harris, C C

Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than normal human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6285955
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:11; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEUPLOIDY
ANIMAL CELLS
ANIMALS
ASBESTOS
BIOLOGICAL EFFECTS
BODY
CARCINOGENESIS
CELL CULTURES
ELECTRON MICROSCOPY
GENETIC EFFECTS
LUNGS
MAMMALS
MAN
MICROSCOPY
ORGANS
PATHOGENESIS
PLOIDY
PRIMATES
RESPIRATORY SYSTEM
RESPIRATORY TRACT CELLS
SCANNING ELECTRON MICROSCOPY
VERTEBRATES