Role of leukocytes in response to acute myocardial ischemia and reflow in dogs

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:6283499

Engler, R L; Dahlgren, M D; Morris, D D; Peterson, M A; Schmid-Schoenbein, G W

Recent evidence indicates that leukocytes (LEU) are large, stiff, viscous cells that naturally adhere to vascular endothelium. The authors perfused the coronary circulation under matched, controlled conditions with whole blood or granulocyte-depleted whole blood. During 1 h of ischemia circumflex perfusion pressure was servocontrolled to a constant value. In whole blood-perfused hearts, flow measured by the radiolabeled microsphere method decreased in endocardium from 0.12 +/- 0.05 at 5 min of ischemia to 0.09 +/- 0.04 ml x min x g at 60 min of ischemia and in epicardium from 0.27 +/- 0.17 to 0.21 +/- 0.16 ml x min x g . In granulocyte-depleted blood-perfused hearts, flow increased over the same period from 0.18 +/- 0.15 to 0.29 +/- 0.18 ml x min x g in endocardium and did not change significantly in epicardium. The LEU-depleted blood perfusate contained less than 33 granulocytes/ l, whereas control perfusate contained 4265/ l. Reperfusion at normal pressures with carbon suspension allowed for histologic evaluation of the no-reflow phenomenon. With whole blood perfusion the no-reflow phenomenon in the endocardium was present with 27% of capillaries occluded, compared with nearly complete reperfusion in LEU-depleted animals. Furthermore, LEU depletion prevented the increases in tissue water content seen in control hearts and decreased the incidence of ventricular arrhythmias. These studies demonstrate the significant participation of granulocytes in the unfavorable responses of flow, edema formation, and arrhythmias to the 1st h of myocardial ischemia and further document their role in the no-reflow phenomenon.

• Research Organization: Univ. of California, La Jolla
• OSTI ID: 6283499
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:2; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FLOW
BODY
BODY FLUIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DIAGNOSIS
DISEASES
DOGS
DYNAMIC FUNCTION STUDIES
HEART
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MICROSPHERES
MUSCLES
MYOCARDIUM
ORGANS
PHYSIOLOGY
VERTEBRATES