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Biodegradation of crude oil in a marine environment. general methodology

Conference · · Am. Chem. Soc., Div. Environ. Chem., Prepr.; (United States)
OSTI ID:6283303
Continuous and batch culture studies of the biodegradation of an Arabian Light crude oil in a 20C, pH 8.1 artificial sea water by a mixed culture of bacteria obtained by enrichment of sediments polluted by the Amoco Cediz oil spill showed that 25% of the total hydrocarbons, mainly saturated, were consumed during the first 20 hr of culture, with little degradation of the aromatic fraction. The over-all biodegradation process slowed after 20 hr, although aromatic hydrocarbon degradation increased. The over-all degradation accounted for 41% of the total hydrocarbons consumed, 67% of the saturated fraction, and 27% of the aromatic fraction. Asphaltenes were not degraded. Gas chromatographic analyses of the saturated fraction indicated the disappearance of n-alkanes and isoprenoid alkanes, and mass spectrometric analyses indicated that some of the one-ring naphthenes disappeared.
OSTI ID:
6283303
Report Number(s):
CONF-800814-
Conference Information:
Journal Name: Am. Chem. Soc., Div. Environ. Chem., Prepr.; (United States) Journal Volume: 20:2
Country of Publication:
United States
Language:
English