Host-pathogen interactions. XVII. Hydrolysis of biologically active fungal glucans by enzymes isolated from soybean cells

Journal Article · Wed Jul 01 04:00:00 EDT 1981 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.68.1.221· OSTI ID:6282068

Cline, K; Albersheim, P

The ability of ..beta..-glucosylase I, a soybean cell wall ..beta..-glucosyl hydrolase, to degrade elicitors of phytoalexin accumulation was studied. Extensive ..beta..-glucosylase I treatment of the glucan elicitor isolated from the mycelial walls of Phytophthora megasperma var. sojae results in hydrolysis of 77% of the glucosidic bonds of the elicitor and destruction of 94% of its activity. Soybean cell walls contain some additional factor, probably one or more additional enzymes, which can assist ..beta..-glucosylase I in hydrolyzing the glucan elicitor. In a single treatment, the mixture of cell wall enzymes hydrolyzed 91% of the glucosidic bonds and destroyed 85% of the activity of the elicitor. The enzymes from soybean cell walls will also hydrolyze elicitor-active oligoglucosides prepared from the mycelial walls of Phytophthora megasperma var. sojae. The active oligoglucosides are more susceptible than the glucan elicitor to hydrolysis by these enzymes. The mixture of cell wall enzymes or ..beta..-glucosylase I, by itself, hydrolyzes more than 96% of the glucosidic bonds and destroys more than 99% of the activity of the oligoglucoside elicitor. Two possible advantages for the existence of these enzymes in the walls of soybean cells are discussed.

• Research Organization: Univ. of Colorado, Boulder
• OSTI ID: 6282068
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 68:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550900 -- Pathology
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
BIOCHEMISTRY
CARBOHYDRATES
CELL CONSTITUENTS
CELL WALL
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DISEASE RESISTANCE
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCINE HISPIDA
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LEGUMINOSAE
LYSIS
ORGANIC COMPOUNDS
PLANT CELLS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS