Host-pathogen interactions. XVII. Hydrolysis of biologically active fungal glucans by enzymes isolated from soybean cells
The ability of ..beta..-glucosylase I, a soybean cell wall ..beta..-glucosyl hydrolase, to degrade elicitors of phytoalexin accumulation was studied. Extensive ..beta..-glucosylase I treatment of the glucan elicitor isolated from the mycelial walls of Phytophthora megasperma var. sojae results in hydrolysis of 77% of the glucosidic bonds of the elicitor and destruction of 94% of its activity. Soybean cell walls contain some additional factor, probably one or more additional enzymes, which can assist ..beta..-glucosylase I in hydrolyzing the glucan elicitor. In a single treatment, the mixture of cell wall enzymes hydrolyzed 91% of the glucosidic bonds and destroyed 85% of the activity of the elicitor. The enzymes from soybean cell walls will also hydrolyze elicitor-active oligoglucosides prepared from the mycelial walls of Phytophthora megasperma var. sojae. The active oligoglucosides are more susceptible than the glucan elicitor to hydrolysis by these enzymes. The mixture of cell wall enzymes or ..beta..-glucosylase I, by itself, hydrolyzes more than 96% of the glucosidic bonds and destroys more than 99% of the activity of the oligoglucoside elicitor. Two possible advantages for the existence of these enzymes in the walls of soybean cells are discussed.
- Research Organization:
- Univ. of Colorado, Boulder, CO (United States)
- DOE Contract Number:
- EY-76-S-02-1246
- OSTI ID:
- 6282068
- Journal Information:
- Plant Physiol.; (United States), Vol. 68:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
DISEASE RESISTANCE
BIOCHEMISTRY
GLYCOSYL HYDROLASES
ENZYME ACTIVITY
PLANT CELLS
CELL WALL
ENZYMATIC HYDROLYSIS
FUNGI
GLYCINE HISPIDA
POLYSACCHARIDES
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ENZYMES
HYDROLASES
HYDROLYSIS
LEGUMINOSAE
LYSIS
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SOLVOLYSIS
550200* - Biochemistry
550900 - Pathology
553000 - Agriculture & Food Technology