Protein structure by solid-state NMR of oriented systems
Thesis/Dissertation
·
OSTI ID:6281462
A method for determining protein backbone structure from angular information obtainable by solid state NMR spectroscopy is presented. Various spin interactions including quadrupole, dipole, and chemical shift interactions and nuclei including /sup 14/N, /sup 15/N, /sup 13/C, and /sup 2/H may be observed. Angularly dependent measurements can be made when the sample has at least one direction of order along the externally applied magnetic field. Several NMR parameters are used to determine the orientation of each peptide plane with respect to the magnetic field vector, B/sub O/, to within a few symmetry related possibilities. The computer program Totlink can then be used to perform the necessary coordinate transformations and to evaluate the possible backbone structures and select for the most chemically reasonable. Experimental /sup 14/N NMR structural studies of the model peptides n-acetyl-d,l-valine, n-acetyl-l-valyl-l-leucine, and l-alanyl-glycyl-glycine and preliminary /sup 14/N NMR results on a large single crystal of orthorhombic lysozyme are presented.
- Research Organization:
- Pennsylvania Univ., Philadelphia (USA)
- OSTI ID:
- 6281462
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL SHIFT
DEUTERIUM
EVEN-ODD NUCLEI
GLYCINE
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUCINE
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NITROGEN 14
NITROGEN 15
NITROGEN ISOTOPES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RESONANCE
SPECTRA
STABLE ISOTOPES
TRACER TECHNIQUES
VALINE
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL SHIFT
DEUTERIUM
EVEN-ODD NUCLEI
GLYCINE
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUCINE
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NITROGEN 14
NITROGEN 15
NITROGEN ISOTOPES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RESONANCE
SPECTRA
STABLE ISOTOPES
TRACER TECHNIQUES
VALINE