In vivo benzodiazepine receptor binding and imaging using (/sup 3/H)-flunitrazepam

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6281370

Ciliax, B J

The use of (/sup 3/H)-flunitrazepam as a ligand to image and measure alterations in benzodiazepine receptors in vivo in rat was investigated. Animals were injected with (/sup 3/H)-flunitrazepam intravenously, arterial samples of (/sup 3/H)-flunitrazepam were obtained and later the animals were sacrificed to assay brain binding. (/sup 3/H)-Flunitrazepam entered brain rapidly and bound to benzodiazepine receptors. A series of rats were lesioned unilaterally with kainic acid in the caudate-putamen several months prior to the infusion of (/sup 3/H)-flunitrazepam. In vivo autoradiography in lesioned rats showed that benzodiazepine binding in the lesioned striatum was significantly decreased compared to the control side and that benzodiazepine binding in globus pallidus and substantia nigra on the side of the lesion was significantly increased as compared to the intact side. The observed changes in benzodiazepine binding were similar to those observed previously in lesioned rats using in vitro techniques. Thus, benzodiazepine receptor regulation could be imaged quantitatively using in vivo binding techniques.

• Research Organization: Michigan Univ., Ann Arbor (USA)
• OSTI ID: 6281370
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
DRUGS
KINETICS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
PSYCHOTROPIC DRUGS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TRANQUILIZERS
TRITIUM COMPOUNDS
VERTEBRATES