Localization of cellulose synthase in Acetobacter xylinum
The cytoplasmic and outer membranes of Acetobacter xylinum (ATCC 53582) were isolated by discontinuous sucrose density ultracentrifugation. Both lysozyme and trypsin were required for efficient crude membrane separation. Primary dehydrogenases and NADH oxidase were used as cytoplasmic membrane markers, and 2-keto-3-deoxy-octulosonic acid was used to identify the outer membranes. Cellulose synthetase activity was assayed as the conversion of radioactivity from UDP-(/sup 14/C)glucose into an alkali-insoluble ..beta..-1,4-D-(/sup 14/C)glucan. The cellulosic nature of the product was demonstrated by enzymatic hydrolysis followed by thin-layer chromatography, and by methylation analysis followed by thin-layer chromatography and gas chromatography-mass spectroscopy. X-ray diffraction analysis indicated that the in vitro product is cellulose II which is in contrast to the in vivo product, namely cellulose I. In addition, no microfibrillar morphology could be observed from negative stained and metal shadowed preparations of the in vitro product.
- Research Organization:
- Texas Univ., Austin (USA)
- OSTI ID:
- 6280746
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Unique regulatory properties of the UDP-glucose:. beta. -1,4-glucan synthetase of Acetobacter xylinum. [Acetobacter xylinum]
Comparison of the mechanism of cellulose biosynthesis in plants and bacteria. [Acetobacter xylinum]
Related Subjects
BACTERIA
CYTOLOGY
TRANSFERASES
BIOLOGICAL LOCALIZATION
CARBON 14 COMPOUNDS
CELL MEMBRANES
CYTOPLASM
ENZYMATIC HYDROLYSIS
GAS CHROMATOGRAPHY
GLUCOSE
LYSOZYME
MASS SPECTROSCOPY
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRYPSIN
ULTRACENTRIFUGATION
X-RAY DIFFRACTION
ALDEHYDES
BIOLOGY
CARBOHYDRATES
CELL CONSTITUENTS
CENTRIFUGATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
COHERENT SCATTERING
DECOMPOSITION
DIFFRACTION
ENZYMES
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
SACCHARIDES
SCATTERING
SEPARATION PROCESSES
SERINE PROTEINASES
SOLVOLYSIS
SPECTROSCOPY
550301* - Cytology- Tracer Techniques