Localization of cellulose synthase in Acetobacter xylinum
Thesis/Dissertation
·
OSTI ID:6280746
The cytoplasmic and outer membranes of Acetobacter xylinum (ATCC 53582) were isolated by discontinuous sucrose density ultracentrifugation. Both lysozyme and trypsin were required for efficient crude membrane separation. Primary dehydrogenases and NADH oxidase were used as cytoplasmic membrane markers, and 2-keto-3-deoxy-octulosonic acid was used to identify the outer membranes. Cellulose synthetase activity was assayed as the conversion of radioactivity from UDP-(/sup 14/C)glucose into an alkali-insoluble ..beta..-1,4-D-(/sup 14/C)glucan. The cellulosic nature of the product was demonstrated by enzymatic hydrolysis followed by thin-layer chromatography, and by methylation analysis followed by thin-layer chromatography and gas chromatography-mass spectroscopy. X-ray diffraction analysis indicated that the in vitro product is cellulose II which is in contrast to the in vivo product, namely cellulose I. In addition, no microfibrillar morphology could be observed from negative stained and metal shadowed preparations of the in vitro product.
- Research Organization:
- Texas Univ., Austin (USA)
- OSTI ID:
- 6280746
- Country of Publication:
- United States
- Language:
- English
Similar Records
In vitro synthesis of cellulose II from a cytoplasmic membrane fraction of Acetobacter xylinum
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Journal Article
·
Thu Oct 01 00:00:00 EDT 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:7027201
Comparison of the mechanism of cellulose biosynthesis in plants and bacteria. [Acetobacter xylinum]
Conference
·
Fri Dec 31 23:00:00 EST 1982
· J. Appl. Polym. Sci.: Appl. Polym. Symp.; (United States)
·
OSTI ID:6851619
Cellulose biosynthesis in Acetobacter xylinum
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:5719029
Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BACTERIA
BIOLOGICAL LOCALIZATION
BIOLOGY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRIFUGATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
COHERENT SCATTERING
CYTOLOGY
CYTOPLASM
DECOMPOSITION
DIFFRACTION
ENZYMATIC HYDROLYSIS
ENZYMES
GAS CHROMATOGRAPHY
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
LYSOZYME
MASS SPECTROSCOPY
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
SACCHARIDES
SCATTERING
SEPARATION PROCESSES
SERINE PROTEINASES
SOLVOLYSIS
SPECTROSCOPY
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRANSFERASES
TRYPSIN
ULTRACENTRIFUGATION
X-RAY DIFFRACTION
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BACTERIA
BIOLOGICAL LOCALIZATION
BIOLOGY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRIFUGATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
COHERENT SCATTERING
CYTOLOGY
CYTOPLASM
DECOMPOSITION
DIFFRACTION
ENZYMATIC HYDROLYSIS
ENZYMES
GAS CHROMATOGRAPHY
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
LYSOZYME
MASS SPECTROSCOPY
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
SACCHARIDES
SCATTERING
SEPARATION PROCESSES
SERINE PROTEINASES
SOLVOLYSIS
SPECTROSCOPY
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRANSFERASES
TRYPSIN
ULTRACENTRIFUGATION
X-RAY DIFFRACTION