In situ hybridization of oxytocin messenger RNA: macroscopic distribution and quantitation in rat hypothalamic cell groups

Journal Article · Fri May 29 00:00:00 EDT 1987 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(87)91280-0· OSTI ID:6280193

Burbach, J P; Voorhuis, T A; van Tol, H H; Ivell, R

Oxytocin mRNA was detected in the rat hypothalamus by in situ hybridization to a single stranded /sup 35/S-labelled DNA probe and the distribution of oxytocin mRNA-containing cell groups was studied at the macroscopic level. Specificity of hybridization was confirmed by comparison to vasopressin mRNA hybridization in parallel tissue sections. Cell groups containing oxytocin mRNA were confined to a set of hypothalamic cell groups, i.c. the supraoptic, paraventricular, anterior commissural nuclei, nucleus circularis and scattered hypothalamic islets. These cell groups displayed similar densities of autoradiographic signals indicating that the oxytocin gene is expressed at approximately the same average level at these various sites.

• Research Organization: Univ. of Utrecht, The Netherlands
• OSTI ID: 6280193
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MESSENGER-RNA
HYBRIDIZATION
TISSUE DISTRIBUTION
AUTORADIOGRAPHY
DNA
GENES
HYPOTHALAMUS
OXYTOCIN
RATS
SULFUR 35
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
EVEN-ODD NUCLEI
HORMONES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
RNA
RODENTS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques