Protein C inhibits endocytosis of thrombin-thrombomodulin complexes in A549 lung cancer cells and human umbilical vein endothelial cells
Journal Article
·
· Blood; (United States)
OSTI ID:6280178
We investigated the effect of protein C on the endocytosis of thrombin-thrombomodulin complexes. We previously showed that exposure of umbilical vein endothelial cells to thrombin stimulated the internalization and degradation of thrombin. A similar internalization was stimulated by a monoclonal antithrombomodulin antibody. We have repeated these studies in the presence of protein C and found that endocytosis of /sup 125/I-thrombin-thrombomodulin complexes, but not /sup 125/I-antithrombomodulin-thrombomodulin complexes, is inhibited. Activated protein C did not inhibit endocytosis of thrombin-thrombomodulin complexes. Protein C inhibited both internalization and degradation of /sup 125/I-thrombin and diisopropylphosphoryl (DIP) /sup 125/I-thrombin in human lung cancer cells (A549). These effects were observed at protein C concentrations found in human plasma. Protein S had no effect on the inhibition of endocytosis of thrombin-thrombomodulin complexes by protein C. We propose that protein C may regulate the rate of endocytosis of thrombin-thrombomodulin complexes in vivo and thereby control the capacity for endothelium to activate protein C.
- Research Organization:
- Washington Univ. School of Medicine, St Louis, MO
- OSTI ID:
- 6280178
- Journal Information:
- Blood; (United States), Journal Name: Blood; (United States) Vol. 5; ISSN BLOOA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Equilibrium binding of thrombin to recombinant human thrombomodulin: Effect of hirudin, fibrinogen, factor Va, and peptide analogues
Covalent binding of human thrombin to a human endothelial cell-associated protein
Presence of plasma proteins facilitates the uptake of /sup 125/I-thrombin by the rabbit thoracic aorta endothelium in vitro
Journal Article
·
Wed Oct 31 23:00:00 EST 1990
· Biochemistry; (USA)
·
OSTI ID:5736518
Covalent binding of human thrombin to a human endothelial cell-associated protein
Journal Article
·
Tue Feb 28 23:00:00 EST 1989
· Experimental Cell Research; (United States)
·
OSTI ID:5623323
Presence of plasma proteins facilitates the uptake of /sup 125/I-thrombin by the rabbit thoracic aorta endothelium in vitro
Journal Article
·
Tue Jul 01 00:00:00 EDT 1986
· Thromb. Res.; (United States)
·
OSTI ID:5013215
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CHEMICAL REACTIONS
COAGULANTS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DIGESTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
FETAL MEMBRANES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTRACELLULAR DIGESTION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LUNGS
MAMMALS
MAN
MEMBRANES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
SERINE PROTEINASES
THROMBIN
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CHEMICAL REACTIONS
COAGULANTS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DIGESTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
FETAL MEMBRANES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTRACELLULAR DIGESTION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LUNGS
MAMMALS
MAN
MEMBRANES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
SERINE PROTEINASES
THROMBIN
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES