Biochemical analysis of plant protection afforded by a nonpathogenic endophytic mutant of Colletotrichum magna
- Geological Survey, Seattle, WA (United States) Univ. of Washington, Seattle, WA (United States). Dept. of Botany
- Geological Survey, Seattle, WA (United States)
- Volcani Center, Bet Dagan (Israel). Dept. of Plant Pathology
A nonpathogenic mutant of Colletotrichum magna (path-1) was previously shown to protect watermelon (Citrullus lanatus) and cucumber (Cucumis sativus) seedlings from anthracnose disease elicited by wild-type C. magna. Disease protection was observed in stems of path-1-colonized cucurbits but not in cotyledons, indicating that path-1 conferred tissue-specific and/or localized protection. Plant biochemical indicators of a localized and systemic (peroxidase, phenylalanine ammonia-lyase, lignin, and salicylic acid) plant-defense response were investigated in anthracnose-resistant and-susceptible cultivars of cucurbit seedlings exposed to four treatments: (1) water (control), (2) path-1 conidia, (3) wild-type conidia, and (4) challenge conditions (inoculation into path-1 conidia for 48 h and then exposure to wild-type conidia). Collectively, these analyses indicated that disease protection in path-1-colonized plants was correlated with the ability of these plants to mount a defense response more rapidly and to equal or greater levels than plants exposed to wild-type C. magna alone. Watermelon plants colonized with path-1 were also protected against disease caused by Colletotrichum orbiculare and Fusarium oxysporum. A model based on the kinetics of plant-defense activation is presented to explain the mechanism of path-1-conferred disease protection.
- Sponsoring Organization:
- National Science Foundation (NSF); USDOE; DOA; National Science Foundation, Washington, DC (United States); USDOE, Washington, DC (United States); Department of Agriculture, Washington, DC (United States)
- OSTI ID:
- 6279795
- Journal Information:
- Plant Physiology; (Bethesda), Vol. 119:2; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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