Down-regulation of protein kinase C in mouse cells and tissues
The process by which protein kinase C (PKC) activity is lost in response to phorbol ester treatment (down regulation) was investigated by immunoblotting electrophoretically separated proteins with PKC-specific chicken egg yolk antibody as well as determination of PKC activity and phorbol ester-binding activity in cellular extracts. Immunoreactive peptides of 66, 61, 50, and 40 kilodaltons were observed in soluble cell extracts 10 min to 12 hours after treatment of C3H 10T 1/2 mouse fibroblasts with phorbol ester. The smaller fragments appeared at the later times while the 66 kDa peptide was almost undetectable after 12 h of treatment. Down-regulation of PKC did not occur after treatment of cells with 1 M dioctanoylglycerol. The question of incomplete down-regulation versus residual kinase-receptor-active fragments was investigated. Modulation of down-regulation and appearance of immunoreactive fragments by protease inhibitors, inhibitors of PKC, and antagonists of promotion of mouse skin tumors also was studied.
- Research Organization:
- Purdue Univ., West Lafayette, IN
- OSTI ID:
- 6279768
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHORBOL ESTERS
BIOCHEMICAL REACTION KINETICS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
ELECTROPHORESIS
ENZYME INHIBITORS
FIBROBLASTS
IMMUNE REACTIONS
MICE
PEPTIDES
TUMOR CELLS
ANIMAL CELLS
ANIMALS
CARCINOGENS
CONNECTIVE TISSUE CELLS
ENZYMES
ESTERS
KINETICS
MAMMALS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
REACTION KINETICS
RODENTS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology