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Effects of chloroquine and hepatic stimulator substance on cellular accumulation and nuclear binding of sup 125 I-epidermal growth factor in primary culture of adult rat hepatocytes

Journal Article · · Research Communications in Chemical Pathology and Pharmacology; (USA)
OSTI ID:6278051
; ; ;  [1]
  1. Tottori Univ. School of Medicine, Yonago (Japan)
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The effects of chloroquine and hepatic stimulator substance (HSS) on cellular accumulation and nuclear binding of {sup 125}I-epidermal growth factor (EGF) were examined in primary culture of adult rat hepatocytes. When intact hepatocytes were incubated at 37{degrees}C with {sup 125}I-EGF, the cellular accumulation and the nuclear binding reached a peak at 1 h and declined thereafter, where the nuclear binding was 2.49% at 1 h and 2.53% at 2 h. Chloroquine resulted in a time-dependent increase in the cellular accumulation and the nuclear binding was 3.37% at 1 h and 3.72% at 2 h. In contrast, HSS produced no change in each value, suggesting that HSS does not modulate EGF receptors in plasma membrane and nucleus.
OSTI ID:
6278051
Journal Information:
Research Communications in Chemical Pathology and Pharmacology; (USA), Journal Name: Research Communications in Chemical Pathology and Pharmacology; (USA) Vol. 69:1; ISSN 0034-5164; ISSN RCOCB
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CELL NUCLEI
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LIVER CELLS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIDINES
QUINOLINES
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SKIN
SOMATIC CELLS
TIME DEPENDENCE
TISSUES
VERTEBRATES