Quantification of cell death in developing cerebellum by a /sup 14/C tracer method
Journal Article
·
· Brain Res. Bull.; (United States)
To study the question of whether or not cell death contributes significantly to normal or stressed postnatal brain development in a way which is biochemically quantifiable, we carried out an experiment to assess the amount of cell death in developing cerebellum. By measuring the loss of DNA content and the loss of /sup 14/C from labelled thymidine previously incorporated into the DNA fraction (DNAF) in X-irradiated neonatal animals, shown by histological methods to have cell death to the degree of degranulating the external granular layer (EGL), we showed that when cells die both label and DNA content are greatly decreased in the cerebellum. Experiments on both normal and malnourished animals showed that cell death does not contribute significantly to cerebellar development in either malnutrition-stressed or normal animals. Here, we present a biochemical tool for assessing cell death and evidence that cell death does not contribute significantly to cerebellar development.
- Research Organization:
- Univ. of Texas, Dallas (USA)
- OSTI ID:
- 6276894
- Journal Information:
- Brain Res. Bull.; (United States), Journal Name: Brain Res. Bull.; (United States) Vol. 3:4; ISSN BRBUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL STRESS
BODY
BRAIN
CARBON 14 COMPOUNDS
CELL DIFFERENTIATION
CENTRAL NERVOUS SYSTEM
CEREBELLUM
DNA
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEONATES
NERVOUS SYSTEM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUTRITION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATIONS
RATS
RIBOSIDES
RODENTS
SURVIVAL TIME
THYMIDINE
TRACER TECHNIQUES
VERTEBRATES
VIABILITY
X RADIATION
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL STRESS
BODY
BRAIN
CARBON 14 COMPOUNDS
CELL DIFFERENTIATION
CENTRAL NERVOUS SYSTEM
CEREBELLUM
DNA
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEONATES
NERVOUS SYSTEM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUTRITION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATIONS
RATS
RIBOSIDES
RODENTS
SURVIVAL TIME
THYMIDINE
TRACER TECHNIQUES
VERTEBRATES
VIABILITY
X RADIATION