Nucleic acid labeling with ( sup 3 H)orotic acid and nucleotide profile in rats in protein deprivation, enteral and parenteral essential amino acid administration, and 5-fluorouracil treatment

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · JPEN, Journal of Parenteral and Enteral Nutrition; (USA)

DOI:https://doi.org/10.1177/0148607190014005490· OSTI ID:6276883

Jakobsson, B; el Hag, I A; Andersson, M; Christensson, P I; Stenram, U ^[1]

---

1. Univ. of Lund (Sweden)

Rats were fed a 0% casein diet for 1 week, with or without enteral or parenteral administration of essential amino acids, or a 25% casein diet, in one group supplemented with 5-fluorouracil treatment. Ninety minutes before sacrifice the rats were given a tracer of (3H)orotic acid. Incorporation into the acid soluble fraction, RNA, and DNA was determined in liver, small intestine, bone marrow, and kidney. Nucleotide profile was examined in liver and intestine. Protein deficiency caused inter alia a decrease in body weight; a decrease in RNA/DNA ratio and an increase in the specific RNA labeling in liver and kidney; an altered nucleotide profile in the liver; an increase in the nucleotide/DNA and RNA/DNA ratios and a decrease in the specific labeling of the acid soluble fraction, RNA, and DNA in the bone marrow. These changes were prevented to the same extent by giving essential amino acids, either orally or intravenously. The minor changes in intestinal nucleotide profile in protein deprivation were prevented to a slightly larger extent by amino acids orally than parenterally. 5-Fluorouracil treatment gave a decrease in the RNA/DNA ratio in the liver and kidney but an increase in the nucleotide/DNA and RNA/DNA ratios in the bone marrow. Nucleotide profiles were unaltered. The amount of DNA per gram of tissue decreased in bone marrow and increased in kidney. Parenteral administration per se resulted in almost no changes.

• OSTI ID: 6276883
• Journal Information: JPEN, Journal of Parenteral and Enteral Nutrition; (USA), Vol. 14:5; ISSN 0148-6071
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CASEIN
NUTRITIONAL DEFICIENCY
DNA
LABELLING
RNA
AMINO ACIDS
BONE MARROW
DIET
FLUOROURACILS
KIDNEYS
LIVER
NUCLEOTIDES
OROTIC ACID
QUANTITY RATIO
RATS
SMALL INTESTINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
AZINES
BODY
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
GLANDS
HEMATOPOIETIC SYSTEM
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INTESTINES
ISOTOPE APPLICATIONS
MAMMALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RODENTS
TISSUES
URACILS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques