Effects of dithiothreitol, a sulfhydryl reducing agent, on CA1 pyramidal cells of the guinea pig hippocampus in vitro
Technical Report
·
OSTI ID:6275944
The radioprotectant, dithiothreitol (DTT) has been shown to increase excitability in the hippocampal slice preparation. In the present study, intracellular recording techniques were used to further examine the actions of DTT. Electrophysiological recordings from CA1 pyramidal cells were examined prior to, during and after DTT exposure. DTT caused a small depolarization without altering membrane resistance. DTT-induced spontaneous firing and occasional burst firing in normally silent neurons. These effects were accompanied by a reduction in spike frequency adaptation but no change in the after hyperpolarization following a train of action potentials. Following DTT exposure, orthodromic stimulation produced multiple firing. subthreshold excitatory postsynaptic potentials (EPSPs) were significantly prolonged. Isolating the CA1 subfield, attenuated the prolongation of the EPSP by DTT. Recurrent inhibitory postsynaptic potentials were unaffected by DTT. The actions of DTT are likely to result from DTT-induced reduction of disulfide bonds since the reduced form of DTT does not cause a similar hyperexcitability.
- Research Organization:
- Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 6275944
- Report Number(s):
- AD-A-203084/9/XAB; AFRRI-SR-88-28
- Country of Publication:
- United States
- Language:
- English
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Wed Dec 31 23:00:00 EST 1986
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OSTI ID:6170443
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Conference
·
Sun Mar 10 23:00:00 EST 1991
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
·
OSTI ID:5905235
Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHALCOGENIDES
CHEMICAL BONDS
DEPOLARIZATION
DRUGS
ELECTROPHYSIOLOGY
ENERGY-LEVEL TRANSITIONS
EXCITATION
HIPPOCAMPUS
NERVE CELLS
NERVOUS SYSTEM
ORGANS
PHYSIOLOGY
RADIATION EFFECTS
RADIOPROTECTIVE SUBSTANCES
RECORDING SYSTEMS
SOMATIC CELLS
SULFIDES
SULFUR COMPOUNDS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHALCOGENIDES
CHEMICAL BONDS
DEPOLARIZATION
DRUGS
ELECTROPHYSIOLOGY
ENERGY-LEVEL TRANSITIONS
EXCITATION
HIPPOCAMPUS
NERVE CELLS
NERVOUS SYSTEM
ORGANS
PHYSIOLOGY
RADIATION EFFECTS
RADIOPROTECTIVE SUBSTANCES
RECORDING SYSTEMS
SOMATIC CELLS
SULFIDES
SULFUR COMPOUNDS