Expression of P-glycoprotein in adult T-cell leukemia cells

Journal Article · Thu Nov 15 04:00:00 EST 1990 · Blood (Journal of Hematology); (USA)

OSTI ID:6275610

Kuwazuru, Y; Hanada, S; Furukawa, T; Yoshimura, A; Sumizawa, T; Utsunomiya, A; Ishibashi, K; Saito, T; Uozumi, K; Maruyama, M ^[1]

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1. Kagoshima Univ. (Japan)

We have examined the expression of P-glycoprotein (P-gp) in adult T-cell leukemia (ATL) samples from 25 patients. Based on immunoblotting with a monoclonal antibody against P-gp, C219, 8 of 20 ATL patients were P-gp positive at the initial presentation. All 6 patients at the relapsed stage were P-gp positive, and refractory to chemotherapy. The expression of MDR1 mRNA in P-gp-positive ATL cells was increased at the relapsed stage of one patient. P-gp of this patient was photolabeled with (3H)azidopine and the labeling was inhibited with nimodipine, vinblastine and progesterone. These results suggest that P-gp expressed in ATL cells from patients at relapsed stage has the same binding site(s) for the drugs as that in multidrug resistant cells, and is correlated with the refractory nature of the cells to chemotherapy.

• OSTI ID: 6275610
• Journal Information: Blood (Journal of Hematology); (USA), Journal Name: Blood (Journal of Hematology); (USA) Vol. 76:10; ISSN BLOOA; ISSN 0006-4971
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMOTHERAPY
CONNECTIVE TISSUE CELLS
DISEASES
GENE REGULATION
GLYCOPROTEINS
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MONOCLONAL ANTIBODIES
NEOPLASMS
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
SOMATIC CELLS
THERAPY