Photoaffinity crosslinking of etorphine with opioid binding sites in the bovine adrenal medulla
Journal Article
·
· Biotechnol. Appl. Biochem.; (United States)
OSTI ID:6274001
The covalent crosslinking of (/sup 3/H)etorphine with opioid binding sites in the bovine adrenal medulla is reported. Of all the radiolabeled opiates tested (ethylketocyclazocine, etorphine, (D-Ala2, D-Leu5)enkephalin, (D-Ala2, Me-Phe4, Gly5-ol)enkephalin only etorphine could be crosslinked under uv irradiation. In our conditions (black uv lamp, 160 W, peak mean 360 nm, from a distance of 10 cm) maximum covalent binding was observed after a 10-min irradiation. Protein concentration was a crucial factor for the irreversible/total binding ratio. A good ratio (50%) was obtained at protein concentrations of about 1.0 mg/ml. Covalent binding of nonmodified opiates could be of interest for the biochemical characterization of their binding sites.
- Research Organization:
- Laboratoire de Neuroendocrinologie Experimentale, Marseille, France
- OSTI ID:
- 6274001
- Journal Information:
- Biotechnol. Appl. Biochem.; (United States), Journal Name: Biotechnol. Appl. Biochem.; (United States) Vol. 9:2; ISSN BABIE
- Country of Publication:
- United States
- Language:
- English
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·
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CROSS-LINKING
DOMESTIC ANIMALS
DRUGS
ENDORPHINS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIATION EFFECTS
REACTION KINETICS
RECEPTORS
RUMINANTS
SOLUBILITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CROSS-LINKING
DOMESTIC ANIMALS
DRUGS
ENDORPHINS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIATION EFFECTS
REACTION KINETICS
RECEPTORS
RUMINANTS
SOLUBILITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES