Kinetics of chlorinated hydrocarbon degradation by suspended cultures of methane-oxidizing bacteria

Journal Article · · Research Journal of the Water Pollution Control Federation; (USA)

OSTI ID:6271909

Strand, S E; Bjelland, M D; Stensel, H D

The kinetics of methane utilization and the biodegradation of trichloroethylene (TCE) and 1,1,1-trichloroethane (TCA) by a mixed, methanotrophic bacterial culture were studied in a closed-system reactor. Methane oxidation followed Michaelis-Menten kinetics. TCE and TCA degradation followed first-order kinetics with rate constants of 3.7 {times} 10{sup {minus}4} and 8.8 {times} 10{sup {minus}5} L/mg VSS {center dot} h, respectively, for concentrations less than 3,000 {mu}g/L. Oxidative activity of the methanotrophic culture ceased at a dissolved TCE concentration of 7,770 {mu}g/L. TCA, but not TCE, biodegradation was inhibited by dissolved methane concentrations in excess of 0.25 mg/L. In the absence of methane, the culture continued to degrade TCE and TCA, but degradation ceased after 104 hours. Lower biodegradation rates were observed when treating a mixture of TCE and TCA.

• OSTI ID: 6271909
• Journal Information: Research Journal of the Water Pollution Control Federation; (USA), Journal Name: Research Journal of the Water Pollution Control Federation; (USA) Vol. 62:2; ISSN RJWFE; ISSN 1047-7624
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
098000* -- Biomass Fuels-- Waste Management-- (1990-)
ALKANES
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DECOMPOSITION
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
METABOLISM
METHANE
METHANOTROPHIC BACTERIA
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDATION