Cellular radiosensitivity in V79 cells is linked to alterations in chromatin structure

Journal Article · Thu Nov 01 04:00:00 EST 1990 · International Journal of Radiation Oncology, Biology and Physics; (USA)

DOI:https://doi.org/10.1016/0360-3016(90)90228-C· OSTI ID:6271386

Gordon, D J; Milner, A E; Beaney, R P; Grdina, D J; Vaughan, A T ^[1]

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1. Univ. of Birmingham (England)

V79 cells grown as spheroids are more radioresistant than those grown as monolayers. Viable cells from spheroid culture contain restraints to ethidium bromide driven rewinding of DNA supercoils that are absent in monolayer cells. Spheroid cells also contain a DNA-protein matrix that is more resistant to detergent-induced degradation. The increase in structural integrity may be related to a 55-60 kD protein in the nucleoids of spheroid, but not monolayer cells. Spheroid cell radioresistance may therefore be related to a more stable chromatin platform for high fidelity repair of DNA damage.

• OSTI ID: 6271386
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (USA), Journal Name: International Journal of Radiation Oncology, Biology and Physics; (USA) Vol. 19:5; ISSN 0360-3016; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CHROMATIN
DNA
DNA REPAIR
HAMSTERS
LUNGS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOSENSITIVITY
RECOVERY
REPAIR
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES