Localization of human colorectal carcinoma xenografts in mice using radiolabeled monoclonal antibody to carcinoembryonic antigen

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Diagn. Clin. Immunol.; (United States)

OSTI ID:6270070

Tsai, L C; Chen, H M; Yeh, S H; Yuan, C C; Tsao, D; Han, S H

A mouse IgG1 monoclonal antibody (MAbC27) with high affinity for human carcinoembryonic antigen (CEA) has been used in vitro and in vivo for study of human colorectal carcinoma (CRC). After introduction to tumor-bearing mice, the distribution of radiolabeled MAbC27 and its F(ab')2 fragments was analyzed by direct measurement of radioactivity in the tumor and in normal organs as well as by whole-body scanning. Radiolabeled MAbC27 and its F(ab')2 were used successfully in localizing tumor xenografts in mice 40 and 20 hr after inoculation, respectively. A tumor as small as 60 mg (4.2 mm in diameter) was visualized by labeled F(ab')2. Circulating CEA, up to 200 micrograms per mouse did not prevent successful tumor imaging. This study indicates that the MAbC27 is highly useful in detection and localization of CRC in mice.

• Research Organization: Veterans General Hospital, Taipei, Taiwan (China)
• OSTI ID: 6270070
• Journal Information: Diagn. Clin. Immunol.; (United States), Journal Name: Diagn. Clin. Immunol.; (United States) Vol. 5:6; ISSN DCIME
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
BODY
CARCINOEMBRYONIC ANTIGEN
CARCINOMAS
COUNTING TECHNIQUES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
EXPERIMENTAL NEOPLASMS
GASTROINTESTINAL TRACT
INTESTINES
LARGE INTESTINE
MAMMALS
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
ORGANS
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RECTUM
RODENTS
SCINTISCANNING
SPECIFICITY
VERTEBRATES