Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Biochemical and physiological characterization of higher plants with reduced photorespiration: Final report for period May 25, 1981-May 24, 1985

Technical Report ·
OSTI ID:6269008
To further understanding of mechanisms which may reduce photorespiration in terrestrial higher plants, this project sought to critically evaluate the reported effects of nuclear gene dosage on the kinetic and structural properties of green leaf ribulose bisphosphate carboxylase/oxygenase (Rubisco). The results dispel earlier reports that nuclear gene dosage qualitatively affects the enzymic and physicochemical properties of leaf Rubisco, provides definitive evidence that proteolysis accounts for the large subunit charge microheterogeneity in higher plant Rubisco, indicate that ligand-induced changes in protein conformation are responsible for the formation of the exchange-inert Rubisco-carboxyarabinitol bisphosphate (CABP) complex, and provides the first experimental evidence for negative cooperativity in the CABP-Rubisco tight-binding reaction. A second objective was to elucidate the mechanisms by which photorespiration is reduced in various naturally occurring C/sub 3/-C/sub 4/ intermediate species of Moricandia, Panicum and Flaveria. The findings indicate that a C/sub 4/ acid-based CO/sub 2/-concentrating mechanism such as in C/sub 4/- or CAM-photosynthesis is not responsible for reducing photorespiration in the intermediate Moricandia and Panicum species, and provide the first direct experimental evidence to suggest that these C/sub 3/-C/sub 4/ species are capable of an enhanced photosynthetic recycling of photorespiratory CO/sub 2/ via Rubisco. 13 refs.
Research Organization:
Nebraska Univ., Lincoln (USA). Dept. of Biochemistry
DOE Contract Number:
AC02-81ER10902; FG02-84ER13215
OSTI ID:
6269008
Report Number(s):
DOE/ER/10902-T1; DOE/ER/13215-1; ON: DE87014015
Country of Publication:
United States
Language:
English

Similar Records

Reduced apparent photorespiration by the C/sub 3/-C/sub 4/ intermediate species, Moricandia arvensis and Panicum milioides
Journal Article · Thu Feb 28 23:00:00 EST 1985 · Plant Physiol.; (United States) · OSTI ID:5806470
Biochemical and physiological characterization of higher plants with reduced photorespiration. Progress report, May 25, 1981-August 1, 1983
Technical Report · Fri Dec 31 23:00:00 EST 1982 · OSTI ID:5701776
Regulation of photorespiration in leaves: evidence that the fraction of ribulose bisphosphate oxygenated is conserved and stoichiometry fluctuates
Journal Article · Mon Mar 31 23:00:00 EST 1986 · Arch. Biochem. Biophys.; (United States) · OSTI ID:5743156

Related Subjects

14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200 -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALFALFA
BACTERIA
BIOLOGICAL VARIABILITY
C4 SPECIES
CARBON-CARBON LYASES
CARBOXY-LYASES
CEREALS
CONFORMATIONAL CHANGES
ELECTROPHORESIS
ENZYMES
FOOD
GRASS
LEGUMINOSAE
LYASES
MICROORGANISMS
PLANTS
RHIZOBIUM
RIBULOSE DIPHOSPHATE CARBOXYLASE
RYE
SPINACH
TWO-DIMENSIONAL ELECTROPHORESIS
VEGETABLES