Proton suicide: general method for direct selection of sugar transport- and fermentation-defective mutants

Journal Article · Wed Oct 31 23:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:6265347

Winkelman, J W; Clark, D P

A positive selection procedure was devised for bacterial mutants incapable of producing acid from sugars by fermentation. The method relied on the production of elemental bromine from a mixture of bromide and bromate under acidic conditions. When wild-type Escherichia coli cells were plated on media containing a fermentable sugar and an equimolar mixture of bromide and bromate, most of the cells were killed but a variety of mutants unable to produce acid from the sugar survived. Among these mutants were those defective in (i) sugar uptake, (ii) the glycolytic pathway, and (iii) the excretion. There were also novel mutants with some presumed regulatory defects affecting fermentation.

• Research Organization: Southern Illinois Univ., Carbondale
• DOE Contract Number: AC02-82ER12095
• OSTI ID: 6265347
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 160:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCONVERSION
BIOLOGICAL PATHWAYS
BROMINE
CARBOHYDRATES
CHEMICAL REACTIONS
CLEARANCE
CYTOLOGICAL TECHNIQUES
DECOMPOSITION
ELEMENTS
ESCHERICHIA COLI
EXCRETION
FERMENTATION
GLYCOLYSIS
HALOGENS
MICROORGANISMS
MUTANTS
NONMETALS
ORGANIC COMPOUNDS
SACCHARIDES
SENSITIVITY