Characterization by enzyme-linked immunosorbent assay of monoclonal antibodies to Pisum and Avena phytochrome

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.74.1.123· OSTI ID:6259624

Cordonnier, M M; Greppin, H; Pratt, L H

Nine monoclonal antibodies to pea (Pisum sativum L.) and 16 to oat (Avena sativa L.) phytochrome are characterized by enzyme-linked immunosorbent assay against phytochrome from six different sources: pea, zucchini (Cucurbita pepo L.), lettuce (Lactuca sativa L.), oat, rye (Secale cereale L.), and barley (Hordeum vulgare L.). All antibodies were raised against phytochrome with a monomer size near 120,000 daltons. Nevertheless, none of them discriminated qualitatively between 118/114-kilodalton oat phytochrome and a photoreversible, 60-kilodalton proteolytic degradation product derived from it. In addition, none of the 23 antibodies tested discriminated substantially between phytochrome - red-absorbing form and phytochrome - far red-absorbing form. Two antibodies to pea and six to oat phytochrome also bound strongly to phytochrome from the other species, even though these two plants are evolutionarily widely divergent. Of these eight antibodies, two bound significantly to all of the six phytochrome preparations tested, indicating that these two may recognize highly conserved regions of the chromoprotein. Since the molecular function of phytochrome is unknown, these two antibodies may serve as unique probes for regions of this pigment that are important to its mode of action. 27 references, 3 figures, 1 table.

• Research Organization: Univ. of Georgia, Athens
• DOE Contract Number: AS09-81ER10925
• OSTI ID: 6259624
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 74; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
BACTERIA
BIOASSAY
CEREALS
DATA
EXPERIMENTAL DATA
GRASS
IMMUNE REACTIONS
INFORMATION
LEGUMINOSAE
MICROORGANISMS
MOLECULAR BIOLOGY
MONOCLONAL ANTIBODIES
NUMERICAL DATA
OATS
ORGANIC COMPOUNDS
PHYTOCHROMES
PIGMENTS
PISUM
PLANTS
PROTEINS
PURIFICATION
RHIZOBIUM