Role of oral nitrate in the nitrosation of ( UC)proline by conventional microflora and germ-free rats
The urinary excretion of N-nitroso-L-(U- UC)proline by conventional microflora and germ free rats was used to assess the role of the gut bacteria and oral nitrate in the endogenous formation of N-nitroso compounds. The formation of nitrosoproline was qualitatively similar in conventional and germfree rats suggesting no involvement of the intestinal flora in this reaction. Furthermore, nitrosamino acid production was similar following the administration of nitrate and (U- UC)proline or (U- UC)proline alone, demonstrating no involvement of exogenous nitrate under the conditions of the experiment. Dietary contamination with nitrate/nitrite was negligible. The results are consistent with the suggestion that nitrate/nitrite reserves in the body are important in the formation of nitrosoproline in vivo.
- Research Organization:
- British Industrial Biological Research Association, Surrey, England
- OSTI ID:
- 6259475
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Vol. 11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON 14 COMPOUNDS
EXCRETION
NITRATES
BIOCHEMISTRY
NITROSO COMPOUNDS
BIOSYNTHESIS
BACTERIA
INTESTINES
PROLINE
RATS
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BODY
CARBOXYLIC ACIDS
CHEMISTRY
CLEARANCE
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PYRROLES
PYRROLIDINES
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques