UN H and SH H cross-relaxation in hydrated proteins
Journal Article
·
· Biophys. J.; (United States)
The frequency dependence of the proton spin-lattice relaxation time T1 of solid hydrated bovine serum albumin and alpha-chymotrypsin has been measured over 4.5 decades in the range 10(4) to 3 X 10(8) Hz mainly by the aid of the field-cycling technique. The comparison between H2O- and D2O-hydrated samples permitted the distinction of exchangeable and unexchangeable protons. In all cases the UN H cross-relaxation dips due mainly to the amide groups have been observed. In addition, in the case of the deuterium exchanged proteins a SH H quadrupole dip appears. The amide groups act as relaxation sinks due to the coupling of the amide proton to UN and adjacent protons. Outside of the dip regions the proton-proton coupling dominates. The fluctuations of the UN H and H H interactions are of a different type. The unexchangeable protons show a T1 dispersion outside of the quadrupole dip regions given by the exceptional power law T1 approximately v0.75 +/- 0.05. It is shown that apart from structural information of the UN spectra, UN H cross-relaxation spectroscopy permits the determination of correlation times in the range 10(-7) s less than tau less than 10(-4)S.
- Research Organization:
- Universitaet Ulm, Germany, F.R.
- OSTI ID:
- 6257865
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 2; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANIMALS
BODY
CATTLE
CHYMOTRYPSIN
COUPLING
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ENDOCRINE GLANDS
ENZYMES
GLANDS
HEAVY WATER
HYDROGEN COMPOUNDS
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NITROGEN 14
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PANCREAS
PEPTIDE HYDROLASES
PROTEINS
RELAXATION TIME
RESONANCE
RUMINANTS
SERINE PROTEINASES
STABLE ISOTOPES
VERTEBRATES
WATER
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANIMALS
BODY
CATTLE
CHYMOTRYPSIN
COUPLING
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ENDOCRINE GLANDS
ENZYMES
GLANDS
HEAVY WATER
HYDROGEN COMPOUNDS
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NITROGEN 14
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PANCREAS
PEPTIDE HYDROLASES
PROTEINS
RELAXATION TIME
RESONANCE
RUMINANTS
SERINE PROTEINASES
STABLE ISOTOPES
VERTEBRATES
WATER