Increase in the amount of adenylate cyclase in rat gastrocnemius muscle after denervation

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(89)90308-1· OSTI ID:6257683

Hashimoto, K; Watanabe, Y; Uchida, S; Yoshida, H

After section of the sciatic nerve, the basal adenylate cyclase (AC) activity in rat gastrocnemius muscle increased 6-7 times per membrane protein and about 2 times per whole muscle in the following 30 or 40 days. The AC activity in the muscle 30 days after denervation was increased about 4 times by folskolin. Calcitonin gene-related peptide (CGRP) also increased the adenylate cyclase activity in the denervated muscle. The binding of (/sup 3/H)-forskolin to cells isolated from gastrocnemius muscle was examined to determine the amount of AC molecules. Inhibition of (/sup 3/H)-forskolin binding by increasing amounts of unlabeled forskolin gave a sigmoid curve with a IC/sub 50/ value of 3/times/10/sup /minus/7/M. Results showed that the number of (/sup 3/H)-forskolin binding sites per cell was higher on the denervated side than on the control side, like the basal AC activity. The IC/sub 50/ values for inhibition by unlabeled forskolin of binding of (/sup 3/H)-forskolin were similar to muscles on the control and denervated sides. These results suggest that an increase in the AC activity induced by denervation was due to an increase in the numbers of AC molecules in the muscle.

• Research Organization: Osaka Univ. Medical School (Japan)
• OSTI ID: 6257683
• Journal Information: Life Sci.; (United States), Vol. 44:24
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CYCLASES
ENZYME ACTIVITY
MUSCLES
BIOCHEMISTRY
PHOSPHOLIPIDS
BIOCHEMICAL REACTION KINETICS
MEMBRANE PROTEINS
NERVES
PEPTIDES
RATS
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
CHEMISTRY
ENZYMES
ESTERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LYASES
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques