Control of cell division and the spatial localization of assembled gene products in Caulobacter crescentus

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:6257006

Nathan, P D

Experiments are described that examine the role of penicillin-binding proteins (PBPs) in the regulation of cell division in Caulobacter crescentus; and the spatial localization of methyl-accepting chemotaxis proteins (MCPs) in C. crescentus swarmer and predivisional cells. In the analysis of PBP function, in vivo and in vitro assays are used to directly label C. crescentus PBPs with (/sup 3/H) penicillin G in wild type strain CB15, in a series of conditional cell division mutants and in new temperature sensitive cephalosporin C resistant mutants PC8002 and PC8003. 14 PBPs are characterized and a high molecular weight PBP (PBP 1B) that is required for cell division is identified. PBP 1B competes for ..beta..-lactams that induce filament formation and may be a high affinity binding protein. A second high molecular weight PBP (PBP 1C) is also associated with defective cell division. The examination of PBP patterns in synchronous swarmer cells reveals that the in vivo activity of PBP 1B and PBP 1C increases at the time that the cell division pathway is initiated. None of the PBPs, however, appear to be differentially localized in the C. crescentus cell. In the analysis of MCP localization, in vivo and in vitro assays are used to directly label C. crescentus MCPs with methyl-/sup 3/H. MCPs are examined in flagellated and non-flagellated vesicles prepared from cells by immunoaffinity chromatography.

• Research Organization: Princeton Univ., NJ (USA)
• OSTI ID: 6257006
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BIOLOGICAL FUNCTIONS
BIOLOGICAL LOCALIZATION
BIOLOGICAL PATHWAYS
CELL DIVISION
CHROMATOGRAPHY
DRUGS
FUNCTIONS
GENE REGULATION
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
MOLECULAR WEIGHT
MUTANTS
ORGANIC COMPOUNDS
PENICILLIN
PROTEINS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS