Erythroid progenitor cells (CFU-E*) from Friend virus-infected mice undergo VVFe suicide in vitro in the absence of added erythropoietin

Journal Article · Fri Nov 01 04:00:00 EST 1985 · Exp. Hematol.; (United States)

OSTI ID:6255384

Del Rizzo, D F; Axelrad, A A

The authors have investigated the effect of VVFe on the survival in suspension of erythropoietin (epo)-independent erythroid progenitor cells (CFU-E*) induced by Friend polycythemia virus (FV). Spleen cells from C3Hf/Bi mice previously infected with FV were exposed to carrier-free VVFe, and the survival of CFU-E* as a function of time in liquid medium was determined from the number of erythroid colonies that developed from these cells seeded in plasma cultures without added epo. The results showed that spleen CFU-E* were highly vulnerable to VVFe. Marrow CFU-E* behaved in a similar manner. The VVFe responsible for their suicide had been presented to the progenitor cells only during the 4-h period of incubation, after which they were washed and plated in excess nonradioactive iron. They therefore conclude that CFU-E* themselves, and not only their progeny, are capable of actively incorporating iron. Under the same conditions in the absence of added epo, the effect of VVFe on the survival of normal spleen or marrow CFU-E could not be assessed because two few normal CFU-E survived the incubation period. Normal bone marrow cells incubated in complete medium containing epo retained their capacity for erythrocytic colony formation, and CFU-E could then be shown to be vulnerable to VVFe. Thus, either the iron-incorporating system of normal CFU-E was inducible by epo, or else epo permitted survival of the CFU-E so that the activity of a constitutive iron-incorporating system could be recognized.

• Research Organization: Univ. of Toronto, Ontario
• OSTI ID: 6255384
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 10; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
CELL KILLING
COLONY FORMATION
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROPOIETIN
EVEN-ODD NUCLEI
HEMATOPOIETIC SYSTEM
INTERMEDIATE MASS NUCLEI
IRON 55
IRON ISOTOPES
ISOTOPES
MAMMALS
MICE
MICROORGANISMS
NUCLEI
ORGANS
PARASITES
RADIATION EFFECTS
RADIOINDUCTION
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN
SPLEEN CELLS
TISSUES
VERTEBRATES
VIRUSES
YEARS LIVING RADIOISOTOPES