Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Multiparameter analysis of transplantable hemopoietic stem cells. I. The separation and enrichment of stem cells homing to marrow and spleen on the basis of rhodamine-123 fluorescence

Journal Article · · Exp. Hematol.; (United States)
OSTI ID:6254612
; ;
A multiparameter cell separative procedure is described that enables normal transplantable hemopoietic stem cells that preferentially home to the marrow of lethally irradiated mice to be enriched and separated from the majority of spleen colony-forming cells that are assayed 13 days after transplantation (CFU-S13). First, bone marrow cells are centrifuged in a discontinuous bovine serum albumin gradient. Low-density cells are harvested and labeled with the supravital cationic fluorochrome rhodamine 123 (Rh123). Labeled cells are analyzed using a fluorescence-activated cell sorter, and cells are sorted on the basis of relative Rh123 fluorescence within a predetermined forward versus 90 degrees red light scatter window that has been optimized for the recovery and enrichment of cells with marrow repopulating ability (MRA). Cells with MRA were characterized by relatively low Rh123 fluorescence and could be separated from a fraction that fluoresced more intensely and contained the majority of CFU-S13 but low MRA. Cells with platelet repopulating ability cofractionate with MRA whereas cells with erythroid repopulating ability remain associated with CFU-S13.
Research Organization:
Cancer Institute, Melbourne, Victoria, Australia
OSTI ID:
6254612
Journal Information:
Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 10; ISSN EXHMA
Country of Publication:
United States
Language:
English

Similar Records

Separation and functional analysis of bone marrow cells separated by rhodamine-123 fluorescence
Journal Article · Wed Dec 31 23:00:00 EST 1986 · Exp. Hematol.; (United States) · OSTI ID:7083016
Isolation of hemopoietic stem cell subsets from murine bone marrow: II. Evidence for an early precursor of day-12 CFU-S and cells associated with radioprotective ability
Journal Article · Thu Dec 31 23:00:00 EST 1987 · Exp. Hematol.; (United States) · OSTI ID:5592068
Bulk enrichment of transplantable hemopoietic stem cell subsets from lipopolysaccharide-stimulated murine spleen
Journal Article · Sat Jan 31 23:00:00 EST 1987 · Exp. Hematol.; (United States) · OSTI ID:6858057

Related Subjects

550300 -- Cytology
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
BODY
BONE MARROW CELLS
CARBOXYLIC ACIDS
CELL FLOW SYSTEMS
CENTRIFUGATION
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DYES
FLUORESCENCE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMMUNOSUPPRESSION
IRRADIATION
LETHAL IRRADIATION
LUMINESCENCE
MAMMALS
MICE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
RADIOINDUCTION
REAGENTS
RHODAMINES
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SPLEEN
VERTEBRATES