Hemodynamic effects on endothelial cell monolayer detachment from vascular prostheses

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Arch. Surg. (Chicago); (United States)

DOI:https://doi.org/10.1001/archsurg.1989.01410040039008· OSTI ID:6254107

Greisler, H P; Endean, E D; Klosak, J J; Ellinger, J; Henderson, S C; Pham, S M; Durham, S J; Showalter, D P; Levine, J; Borovetz, H S

The establishment of an early blood-contacting endothelialized surface may improve the graft-host relationship. This study evaluated the adherence of indium 111-radiolabeled endothelial cells that were cultured to confluence on fibronectin-treated polyester elastomer (Hytrel) grafts that were perfused for two hours on a pulse duplicator apparatus under high- and low-shear conditions. Perfusate samples were serially assayed for radioactivity. After perfusion, grafts were sectioned into four segments and assayed for retained radioactivity. All graft segments were hematoxylin stained and examined under light microscopy for evaluation of cell density. Excellent endothelial cell adherence (90%) was observed under both hemodynamic conditions at 120 minutes, with most losses occurring within the first 15 minutes. No differences were seen between high- and low-shear conditions or proximal vs distal graft segments.

• Research Organization: Loyola Univ. Medical Center, Maywood, IL (USA)
• OSTI ID: 6254107
• Journal Information: Arch. Surg. (Chicago); (United States), Vol. 124:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ADHESION
BLOOD VESSELS
GRAFTS
BLOOD FLOW
BLOOD PRESSURE
CELL CULTURES
CULTURE MEDIA
DOGS
ENDOTHELIUM
GRAFT-HOST REACTION
INDIUM 111
PERFUSED TISSUES
POLYESTERS
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
POLYMERS
RADIOISOTOPES
TISSUES
TRANSPLANTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques