Ultraviolet-B (290-320 nm)-irradiation inhibits epidermal growth-factor binding to mammalian cells
Mitogens, such as polypeptide growth factors and phorbol ester tumor promoters, act by binding to specific receptors and inducing a pleiotropic response in cultured mammalian cells, which results in the induction of cellular proliferation. An early effect of such agents is the inhibition of binding of epidermal growth factor (EGF) to its receptor. Ultraviolet radiation has also been shown to induce a proliferative response in vivo and in vitro and to act as a tumor promoter in animal skin. We, therefore, examined the effect of ultraviolet radiation (UVB - 290-320 nm) on EGF binding to cells in culture. We found that UVB (100-300 J/m2) induced a rapid, dose-dependent inhibition of EGF binding in a mouse fibroblast cell line, which resulted from a decrease in both number and affinity of binding sites. Phosphorylation of the EGF receptor by protein kinase C (PKC) is not likely to be the mechanism for inhibition, since UVB treatment did not result in PKC activation or modulation of phorbol diester binding.
- Research Organization:
- Columbia Univ., New York, NY (USA)
- OSTI ID:
- 6253601
- Journal Information:
- J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 92:4; ISSN JIDEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARCINOGENS
CELL CULTURES
CELL PROLIFERATION
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
EPIDERMIS
EPITHELIUM
ESTERS
FIBROBLASTS
GROWTH FACTORS
KINETICS
MEMBRANE PROTEINS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECEPTORS
SKIN
SOMATIC CELLS
TISSUES
TRANSFERASES
ULTRAVIOLET RADIATION