Physical mapping of homologous segments of mitochondrial episomes from S male-sterile maize

Journal Article · Thu Dec 31 23:00:00 EST 1981 · Plasmid; (United States)

DOI:https://doi.org/10.1016/0147-619X(82)90022-1· OSTI ID:6253600

Kim, B D ^[1]; Mans, R J; Conde, M F; Pring, D R; Levings, III, C S

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1. Univ. of Florida, Gainesville

Restriction endonuclease maps of two double-stranded plasmid-like DNAs, 6180 and 5175 bp each, isolated as linear molecules from the mitochondria of S-type cytoplasmic male-sterile maize were prepared. Twelve cleavage sites were mapped in each using HindIII, XhoI, EcoRI, SacI, XbaI, SalI, BamHI, and BstEII. BamHI does not cleave S-1 DNA and SalI does not cleave S-2 DNA. A 1150-bp homologous sequence in addition to the 200-bp terminal inverted repeats was terminally oriented on both DNAs by reciprocal hybridization and heteroduplex analysis.

• OSTI ID: 6253600
• Journal Information: Plasmid; (United States), Journal Name: Plasmid; (United States) Vol. 7; ISSN PLSMD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGY
CELL CONSTITUENTS
CEREALS
DNA
ELECTROPHORESIS
ENZYMES
ESTERASES
GENETIC MAPPING
GENETICS
GRAMINEAE
GRASS
HYDROLASES
MAIZE
MAPPING
MITOCHONDRIA
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHODIESTERASES
PLANTS
PLASMIDS