Effect of chlorpromazine on rat arterial lipid synthesis, in vitro

Journal Article · Fri Oct 01 04:00:00 EDT 1982 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02534649· OSTI ID:6253512

Bell, F P; Hubert, E V

The effect of chlorpromazine, a major tranquilizer, on arterial lipid metabolism was studied in vitro in rat aortas incubated with (/sup 14/C)acetate and (/sup 14/C)mevalonate as lipid precursors. Chlorpromazine at a level of 0.25 mM in the incubation medium significantly reduced the incorporation of (/sup 14/C)acetate into free fatty acids (p less than 0.01) and total phospholipids (p less than 0.001) but not triglycerides. Chlorpromazine also altered the pattern of arterial phospholipids synthesized from (/sup 14/C)acetate by significantly increasing the relative proportion of phosphatidylinositol plus phosphatidylserine (p less than 0.02) and reducing the relative proportion of sphingomyelin (p less than 0.001). (/sup 14/C) Acetate incorporation into the combined fractions of steryl esters plus hydrocarbons and sterols plus diglycerides was also significantly reduced (p less than 0.001) by 0.25 mM chlorpromazine. Studies with (/sup 14/C)mevalonate showed that chlorpromazine is also an inhibitor of sterol biosynthesis in arterial tissues as evidenced by 35-40% reductions (p less than 0.05) in the formation of /sup 14/C-labeled squalene and C27 sterols.

• Research Organization: Diabetes and Athersclerosis Research, The Upjohn Company, Kalamazoo, MI
• OSTI ID: 6253512
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 17:10; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AORTA
ARTERIES
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BLOOD VESSELS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHLORPROMAZINE
DRUGS
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
METABOLISM
MOLECULAR BIOLOGY
NORTH AMERICA
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHENOTHIAZINES
PSYCHOTROPIC DRUGS
RATS
REACTION KINETICS
RESEARCH PROGRAMS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRANQUILIZERS
USA
VERTEBRATES