Lung retention and binding of (/sup 14/C)-1-nitropyrene when inhaled by F344 rats as a pure aerosol or adsorbed to carbon black particles

Journal Article · Sun Jan 01 04:00:00 EST 1989 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398909531256· OSTI ID:6251976

Wolff, R K; Sun, J D; Barr, E B; Rothenberg, S J; Yeh, H C

1-Nitropyrene (NP), as found in the environment, is more typically associated with carbonaceous particles than found as an aerosol of the pure compound. To determine whether (and why) an association with particles resulted in prolonged lung retention of NP, rats were exposed to 14C-NP as a pure aerosol or adsorbed on carbon black particles. Total 14C retained in the lung was greater at all times from 0.5 h to 30 d after exposure to 14C-NP adsorbed to carbon black particles than after exposure to pure 14C-NP (p less than .05). The fraction of total 14C in lung bound to carbon black particles decreased steadily with time after exposure, indicating in vivo removal of NP from the particles. At 0.5 h after exposure, the fraction of the estimated deposited 14C that was covalently bound to lung macromolecules was twofold greater for NP adsorbed on carbon black than for pure NP. Covalently bound 14C in lungs increased with time after exposure to 14C-NP adsorbed to carbon black, reaching levels of approximately 1% of the deposited radioactivity at 7-30 d after exposure, whereas levels of covalently bound 14C declined with time after exposure to pure NP. Thus, at 30 d after exposure, the amount of 14C covalently bound to lung macromolecules was approximately 10-fold greater (p less than .05) in rats that inhaled 14C-NP adsorbed on carbon black particles than in rats that inhaled pure 14C-NP aerosols. These results suggest that association of NP with carbon black particles augments the interaction of reactive metabolites of NP with target macromolecules. This phenomenon is thought to be related to the slow release of NP from carbon black particles, and may augment the biological effects of inhaled NP when adsorbed on carbon black or similar particles in the environment.

• Research Organization: Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (USA)
• OSTI ID: 6251976
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 26:3; ISSN JTEHD
• Country of Publication: United States
• Language: English

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADSORPTION
AEROSOLS
ANIMALS
AROMATICS
BODY
CARBON
CARBON 14 COMPOUNDS
CARBON BLACK
CHEMICAL BONDS
COLLOIDS
CONDENSED AROMATICS
COVALENCE
DISPERSIONS
ELEMENTS
HYDROCARBONS
INHALATION
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
METABOLISM
METABOLITES
NITRO COMPOUNDS
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARTICLE SIZE
PYRENE
RATS
RESPIRATORY SYSTEM
RETENTION
RODENTS
SIZE
SOLS
SORPTION
SORPTIVE PROPERTIES
SURFACE PROPERTIES
TRACER TECHNIQUES
VERTEBRATES