Colocalization of neurotensin receptors and of the neurotensin-degrading enzyme endopeptidase 24-16 in primary cultures of neurons

Journal Article · Sat Dec 01 04:00:00 EST 1990 · Journal of Neuroscience; (USA)

OSTI ID:6250622

Chabry, J; Checler, F; Vincent, J P; Mazella, J ^[1]

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1. Institut de Pharmacologie Moleculaire et Cellulaire Centre National de la Recherche Scientifique, Valbonne (France)

This paper compares the localization of neurotensin receptors and of endopeptidase 24-16, a peptidase likely involved in the inactivation of neurotensin in primary cultures of neurons. Neurotensin binding sites were radiolabeled with {sup 125}I-Tyr3-neurotensin, whereas endopeptidase 24-16 was stained by immunohistochemical techniques using a monospecific polyclonal antibody. Endopeptidase 24-16 is present in 80-85% of the nondifferentiated neurons. The proportion of immunoreactive neurons decreased during maturation to reach 35-40% after 4-8 d of culture. By contrast, neurotensin receptors were not detectable in nondifferentiated cells and appear during maturation. Specific {sup 125}I-Tyr3-neurotensin labeling is maximal after 4 d of culture and is located on about 10% of differentiated neurons. Double-labeling experiments show that about 90% of cortical, hypothalamic, and mesencephalic neurons bearing the neurotensin receptor also contained endopeptidase 24-16, supporting the hypothesis that one of the functions of endopeptidase 24-16 is the physiological inactivation of neurotensin. However, the presence of endopeptidase 24-16 on numerous neurons that do not contain neurotensin receptors also suggests that the enzyme could be involved in the degradation and/or maturation of other neuropeptides.

• OSTI ID: 6250622
• Journal Information: Journal of Neuroscience; (USA), Journal Name: Journal of Neuroscience; (USA) Vol. 10:12; ISSN JNRSD; ISSN 0270-6474
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL LOCALIZATION
BODY
BRAIN
CELL DIFFERENTIATION
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FUNCTIONS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLING
MAMMALS
MEMBRANE PROTEINS
MICE
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES