Distribution of mu, delta, and kappa opioid receptor binding sites in the brain of the one-day-old domestic chick (Gallus domesticus): An in vitro quantitative autoradiographic study

Journal Article · Sat Dec 15 04:00:00 EST 1990 · Journal of Comparative Neurology; (USA)

DOI:https://doi.org/10.1002/cne.903020310· OSTI ID:6250426

Csillag, A; Bourne, R C; Stewart, M G ^[1]

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1. Open Univ., Milton Keynes (England)

Three highly specific opioid ligands--(D-Ala2,Gly-ol)-enkephalin (DAGO) for mu (mu) receptor sites, (D-Pen2,D-Pen5)-enkephalin (DPDPE) for delta (delta) sites, and U-69593 for kappa (kappa) sites--were used to determine the regional distribution of the three major subtypes of opioid receptor binding sites in the brains of 1-day-old domestic chicks by the technique of quantitative receptor autoradiography. While there was a degree of heterogeneity in the binding levels of each of the ligands, some notable similarities existed in the binding of the mu and kappa ligands in several forebrain regions, and in the optic tectum of the midbrain where mu and delta binding was very high. In the forebrain there was a high level of binding of mu and kappa ligands in the hyperstriatum, and for the mu ligand there was a very distinct lamination of binding sites in hyperstriatum accessorium, intercalatum supremum, dorsale and ventrale. Levels of binding of the mu and kappa ligands were also high in nucleus basalis, and (for mu only) in the neostriatum. The distribution of binding of the delta specific ligand in the forebrain showed marked differences to that of mu and kappa, being particularly low in the hyperstriatum and neostriatum. Very high levels of labelling of delta binding sites were, however, found in the nucleus rotundus. Binding of the three ligands was generally low or absent in the cerebellum and medulla, apart from a distinct labelling of the granule cell layer by the mu-ligand. A kinetic analysis was made of the binding of the three ligands to whole forebrain sections using scintillation counting methods.

• OSTI ID: 6250426
• Journal Information: Journal of Comparative Neurology; (USA), Journal Name: Journal of Comparative Neurology; (USA) Vol. 302:3; ISSN 0021-9967; ISSN JCNEA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHICKENS
COUNTING TECHNIQUES
DISTRIBUTION
DRUGS
ENDORPHINS
ENKEPHALINS
FOWL
HYDROGEN COMPOUNDS
KINETICS
LIGANDS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
REACTION KINETICS
RECEPTORS
SCINTILLATION COUNTING
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
VERTEBRATES