Recycling of epidermal growth factor-receptor complexes in A431 cells: Identification of dual pathways

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Journal of Cell Biology; (USA)

DOI:https://doi.org/10.1083/jcb.112.1.55· OSTI ID:6249498

Sorkin, A; Krolenko, S; Kudrjavtceva, N; Lazebnik, J; Teslenko, L; Soderquist, A M; Nikolsky, N ^[1]

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1. Institute of Cytology, Leningrad (USSR)

The intracellular sorting of EGF-receptor complexes (EGF-RC) has been studied in human epidermoid carcinoma A431 cells. Recycling of EGF was found to occur rapidly after internalization at 37{degrees}C. The initial rate of EGF recycling was reduced at 18{degrees}C. A significant pool of internalized EGF was incapable of recycling at 18{degrees}C but began to recycle when cells were warmed to 37{degrees}C. The relative rate of EGF outflow at 37{degrees}C from cells exposed to an 18{degrees}C temperature block was slower (t1/2 approximately 20 min) than the rate from cells not exposed to a temperature block (t1/2 approximately 5-7 min). These data suggest that there might be both short- and long-time cycles of EGF recycling in A431 cells. Examination of the intracellular EGF-RC dissociation and dynamics of short- and long-time recycling indicated that EGF recycled as EGF-RC. Moreover, EGF receptors that were covalently labeled with a photoactivatable derivative of {sup 125}I-EGF recycled via the long-time pathway at a rate similar to that of {sup 125}I-EGF. Since EGF-RC degradation was also blocked at 18{degrees}C, we propose that sorting to the lysosomal and long-time recycling pathway may occur after a highly temperature-sensitive step, presumably in the late endosomes.

• OSTI ID: 6249498
• Journal Information: Journal of Cell Biology; (USA), Journal Name: Journal of Cell Biology; (USA) Vol. 112:1; ISSN 0021-9525; ISSN JCLBA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BODY
CARCINOMAS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LYSOSOMES
MAMMALS
MAN
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
SKIN
TEMPERATURE EFFECTS
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES